Imaging with SpheroMatrices® offers distinct advantages over competing solutions.
Current technologies for imaging 3D spheroids/organoids stain these models in whole mount format, i.e. imaging the whole spheroid. This creates issues with light scattering and fluorescence quenching. Furthermore, only targets on the surface of the spheroid can be accessed. As a result, imaging of whole mount spheroids address only very basic questions, such as: are the cells alive and growing, or dying/dead.
SpheroMatrices overcomes these difficulties by making serial sections through the spheroid and can thus access the entire tissue. In addition, as SpheroMatrices uses fixed (preserved) sections, histological methods developed specifically for fixed sections can be employed. This facilitates multiplexed staining, allowing for more complex mechanistic investigations than are available with whole mount format.
Please see our SpheroMatrices flyer for more details.
on an interactive, step by step basis. Collaborations can begin with pilot projects which can then be scaled up to larger research programs, according to your requirements.